Edited by Robert Burns

Plant diseases can have an enormous impact on our lives. In a world where total crop failure can quickly lead to human misery and starvation, accurate diagnostics play a key role in keeping plants free from pathogens. In Plant Pathology: Techniques and Protocols, expert researchers provide methods which are vital to the diagnosis of plant diseases across the globe, addressing all three categories of plant pathology techniques: traditional, serological, and nucleic acid. Chapters examine recent and developing issues with crop identity and authenticity, allowing workers to genotype samples from two major food groups. Each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a notes section which shares tips on troubleshooting and avoiding known pitfalls. Authoritative and reader-friendly, Plant Pathology: Techniques and Protocols is an incredible guide which will be indispensable, both to novices and expert researchers alike.

• Provides researchers with innovative methodologies for plant pathogen diagnostics

• Written by experts from internationally renowned institutions

• Cross-cutting approach to diagnostics, including molecular, serological, and traditional methodologies

• All classes of plant pathogen covered in methodologies

• Provides methods for crop plant genetic validation

Contents

Preface
Contributors

Chapter 1: Bioassay for Diagnosis of Plant Viruses

Chapter 2: Development of Enzyme Linked, Tissue Blot and Dot Blot Immunoassays for Plant Virus Detection

Chapter 3: Immunisation Strategies for Antibody Production

Chapter 4: Preparation of Immunogens and Production of Antibodies

Chapter 5: Enzyme-Linked Immunosorbent Assay Detection of Ralstonia solanacearum in Potatoes: The South African Experience

Chapter 6: Production of Monoclonal Antibodies to Plant Pathogens

Chapter 7: Enzyme-Linked Immunosorbent Assay for the Detection and Identification of Plant Pathogenic Bacteria

Chapter 8: Indirect Immunofluorescence Microscopy for the Detection and Identification of Plant Pathogenic Bacteria

Chapter 9: Detection of Four Major Bacterial Potato Pathogens

Chapter 10: Erwinia amylovora: Modern Methods for Detection and Differentiation

Chapter 11: The Use of Fluorescent In Situ Hybridization in Plant Fungal Identification and Genotyping

Chapter 12: Use of Molecular Methods for the Detection of Fungal Spores

Chapter 13: Identification of Phytophthora fragariae var. rubi by PCR

Chapter 14: Detection of Double-Stranded RNA Elements in the Plant Pathogenic Fungus Rhizoctonia solani

Chapter 15: Immunocapture-PCR for Plant Virus Detection

Chapter 16: Multiplex Polymerase Chain Reaction (PCR) and Real-Time Multiplex PCR for the Simultaneous Detection of Plant Viruses

Chapter 17: Fluorescent-Based Techniques for Viral Detection, Quantification, and Characterization

Chapter 18: Analysis of Population Structures of Viral Isolates Using Single-Strand Conformation Polymorphism Method

Chapter 19: Direct Detection of Plant Viruses in Potato Tubers Using Real-Time PCR

Chapter 20: An Accelerated Soil Bait Assay for the Detection of Potato Mop Top Virus in Agricultural Soil

Chapter 21: Detection of Phytoplasmas of Temperate Fruit Trees

Chapter 22: PCR Detection of Potato Cyst Nematode

Chapter 23: Potato Cultivar Genome Analysis

Chapter 24: Barley Variety Identification Using SSRs

Index

2009; 7.5" x 10.3" hardcover; 326 pages; 42 illustrations; (3 pounds); Item No.

97990$109.00